New method for estimating digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro
نویسندگان
چکیده
منابع مشابه
New method for estimating digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro.
We describe a method by which phagocytosis and digestion of Paracoccidioides brasiliensis yeast cells by polymorphonuclear leukocytes or other phagocytic cells may be estimated. Suspensions of P. brasiliensis in its yeastlike phase were sonicated, counted, and incubated with known numbers of peripheral blood polymorphonuclear leukocytes. At given intervals, cytocentrifuge droplets were stained ...
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In vitro susceptibility testing of Paracoccidioides brasiliensis to sulfonamides.
A total of 60 clinical isolates of Paracoccidioides brasiliensis were tested for susceptibility to sulfadiazine and sulfadimethoxyne by the agar dilution technique. A modification of the Mueller-Hinton medium was devised which gave good growth of the yeast form. The minimum inhibitory concentrations for only 51.6% of the isolates were in the range of the recommended blood serum concentration (5...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1979
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.10.3.365-370.1979